What is rhodamine 123 used for?
Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells.
Where does rhodamine 123 stain in the cell?
mitochondria
Rh-123, a cationic fluorescent dye, accumulates selectively in the mitochondria of eukaryotic cells.
Can rhodamine be used in living cells?
across the mitochondrial membrane may be the basis for the selective staining of mitochondria by rhodamine 123 in living cells.
How do you store rhodamine?
Rhodamine tubulin concentrations <10 mg/ml appear to be less stable and denature when stored for long periods (>2 months). Make single-use aliquots (5–6 µl), flash freeze, and store in liquid nitrogen.
What is mitochondrial membrane potential assay?
The Incucyte® Mitochondrial Membrane Potential Assay enables real-time detection of transient and long-term changes in MMP in live cells and can be multiplexed with cell labeling, apoptosis, or cytotoxicity reagents for additional measurements of cell health.
What does MitoTracker stain?
MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential.
What does propidium iodide measure?
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference.
How toxic is rhodamine?
For Rhodamine B, the effective and lethal concentration (EC50 and LC50) –causing 50% toxicity were in the range of 14–24 mg/L. For Rhodamine WT, no statistically significant effects were observed (p<0.05) at the tsted concentrations (up to 91, 100 and 200 mg/L for algae, crustaceans and fish embryos, respectively).
Why would a doctor order a flow cytometry?
Healthcare providers use flow cytometry to predict how aggressive the cancer will be and to help determine if the cancer will respond to certain treatment. It can also tell you if disease has relapsed (returned) after treatment.
How is mitochondrial health measured?
Oxygen consumption ratio (OCR), maximal oxygen consumption and mitochondrial reserve capacity are good indicators of mitochondrial function. To measure this you need an oxygen electrode and the use of inhibitors such as oligomycin, FCCP, etc.
What does JC-1 reveal about mitochondria?
JC-1, a cationic carbocyanine dye (green) exhibits potential-dependent accumulation in mitochondria where it starts forming J aggregates (red); upon depolarization, it remains as monomer showing green fluorescence.
Can a MitoTracker be fixed?
For MitoTracker® Red CMXRos, we have found that fixing with 3.7% formaldehyde in complete growth medium at 37°C for 15 minutes works well for endothelial cells. 2.3 Rinsing the cells. After fixation, rinse the cells several times in buffer.
Why is MitoTracker used?
However, MitoTracker is chemically reactive, linking to thiol groups in the mitochondria. The dye becomes permanently bound to the mitochondria, and thus remains after the cell dies or is fixed. In addition, it can be used in experiments in which multiple labeling diminishes mitochondrial function.
Is propidium iodide toxic?
CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR 1910.1200. Harmful if swallowed. Possible risk of irreversible effects. Irritating to eyes, respiratory system and skin.
Is rhodamine harmful to humans?
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. The toxicological properties of this substance have not been fully investigated. Inhalation: Causes respiratory tract irritation.
Is Rhodamine B toxic to humans?
A textile dye, Rhodamine B is toxic to the human body. It often enters the body when mixed with food, thereby causing oxidative stress on cells and tissues.