What is SSP PCR?
Sequence-specific amplification (SSP) is simply a form of polymerase chain reaction (PCR) which involves designing one or both primers so that they will or will not allow amplification (the 3′-mismatch principle). Its origins are probably legion, i.e. many people probably thought of it at the same time.
What is SSP and SSO?
• Sequence-Specific Primer (SSP): deceased donors, ambiguity resolution. • Sequence-Specific Oligonulceotide (SSO): routine method.
How does sequence based typing work?
Sequence-based typing involves PCR amplification and sequencing of specific HLA exons, which are known to be polymorphic, from genomic DNA. For each HLA locus both alleles are amplified and sequenced; therefore, it is not always possible to determine exactly which two alleles were responsible for sequence results.
What is PCR SSO?
PCR-SSO is a useful tool that has the advantages of high specificity, sensitivity, and low sample size. This technology makes use of isotopic or non-radiolabeled probes to hybridize the target fragment from PCR amplification, deciding the individual genotype by virtue of positive spots.
What is SSO HLA?
Human leukocyte antigen (HLA) typing, utilising the sequence-specific oligonucleotide (SSO) and sequence-specific primer (SSP) technologies, has been in routine use in many tissue typing laboratories worldwide for more than 20 years since the development of the polymerase chain reaction.
How is HLA typing done?
The blood or cheek swab is tested in a lab to figure out your HLA type. Your HLA type will be compared to potential donors to see if there is a match. Your doctor will decide which donor has the best HLA match for you.
What is molecular HLA typing?
HLA antigens are cell membrane glycoproteins with key roles in the initiation of the immune response. Current methods for HLA typing define HLA alleles and allele groups using DNA-based methods. Different DNA-based molecular techniques are used depending on the clinical application.
What is the difference between real-time PCR and multiplex PCR?
Real-time PCR is a molecular tool for nucleic acid amplification monitored as the reaction progresses. Multiplex PCR technique can use fluorescence to detect, quantitate, and visualize PCR products on a computer monitor by utilizing numerous primer sets.
What is SSO typing?