What is a SpeedVac used for?
Thermo Scientific Savant SpeedVac vacuum concentrators, refrigerated vapor traps and pumps use state-of-the-art centrifugation, vacuum and heat technologies for removing solvents and concentrating samples while maintaining sample integrity.
What does a vacuum concentrator do?
Used in proteomics, genomics, cell biology, microbiology, and drug development, a vacuum centrifuge concentrator uses a vacuum, centrifugal force, and sometimes heat or gas blow down to evaporate liquid and concentrate DNA, RNA, nucleotides, and other proteins for further use or study.
What is a SpeedVac concentrator?
SpeedVac Vacuum Concentrators use built-in chamber heaters. Some models are equipped with radiant lamp on the lid to provide additional heat and prevent condensation of the solvents.
How does a vacuum centrifuge work?
Centrifugal concentrators work by removing solvent under vacuum, so they’re effectively boiling the solvent at a very low temperature. As the pressure in a system decreases, the boiling point of a solvent also decreases, allowing the solvent to boil and turn to a gas at a very low temperature.
What is a sample concentrator?
A sample concentrator is a fast and convenient way of concentrating multiple samples in a block heater at once. Utilising a simple gas delivery system the sample concentrator passes gas over the surface of your samples via stainless steel needles.
What is a Vacufuge?
The Basic Vacufuge is for connecting to an existing vacuum pump. The Complete System includes an integrated PTFE pump and condensation trap. Pump is solvent-resistant and maintenance and oil-free.
Why vacuum is needed in ultracentrifugation?
The ultracentrifuge rotor usually works at 30,000 r/min or more, so centrifuge chamber must maintain a high vacuum to reduce friction of rotor and air.
How does a vacuum evaporator work?
The vacuum evaporation treatment process consists of reducing the interior pressure of the evaporation chamber below atmospheric pressure. This reduces the boiling point of the liquid to be evaporated, thereby reducing or eliminating the need for heat in both the boiling and condensation processes.
What is ultracentrifuge principle?
Principle of Ultracentrifuge : The ultracentrifuge works on the same principle as all other centrifuges. The working of an ultracentrifuge is based on the sedimentation principle, which states that the denser particles settle down faster when compared to less dense particles under gravity.
What is the basic principle of ultracentrifugation?
The Principle of Ultracentrifugation The basis of ultracentrifugation is the same as normal centrifugation: to separate the components of a solution based on their size and density, and the density (viscosity) of the medium (solvent) (Ohlendieck & Harding, 2017).
What is the difference between centrifuge and ultracentrifuge?
One of the frequently employed and most useful techniques in the Molecular Biology Laboratory is centrifugation. On the other hand, ultracentrifugation uses a high-speed centrifuge to determine larger molecules’ molecular weights or separate colloidal and other small particles and determine their size.
What is ultracentrifugation used for?
Accordingly, ultracentrifugation is commonly used to purify, as well as characterize, low-molecular weight polymers up to multi-megaDalton protein complexes and organelles.
What is difference between centrifugation and ultracentrifugation?
Centrifugation Versus Ultracentrifugation: There is a vast difference in the speed of the rotors of centrifuge and ultracentrifuge as they rotate. An ultracentrifuge rotor can spin as high as 1000000 x g, while the most common centrifuges have a relatively lesser speed.