What is in Phusion GC buffer?
The 5x Phusion GC Buffer contains 7.5 mM MgCl2, which provides 1.5 mM MgCl2 in final reaction conditions. Both Phusion Buffers supply 1.5 mM MgCl2 at final reaction conditions. If higher MgCl2 concentrations are desired, use 50 mM MgCl2 solution to increase the MgCl2 titer.
What is Phusion High-Fidelity DNA Polymerase?
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available.
What does GC buffer do in PCR?
However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
What does Hot Start PCR do?
Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation. Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence without an additional temperature-sensitive reaction activation component.
What is Phusion Flash?
Phusion Flash DNA Polymerase is a proofreading polymerase that contains a unique processivity-enhancing domain, making this polymerase accurate and rapid. Phusion Flash DNA Polymerase is a hot start polymerase utilizing a reversibly binding Affibody® protein.
What is Phusion DNA Polymerase?
Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates.
What is the function of DMSO?
Dimethyl sulfoxide (DMSO) is an organic polar aprotic molecule with an amphipathic nature that is ideal for dissolving poorly soluble polar and non-polar molecules. DMSO is widely used as solvent in toxicology and pharmacology, for cryopreservation of cells, and as penetration enhancer during topological treatments.
Why do we use DMSO?
DMSO is used topically to decrease pain and speed the healing of wounds, burns, and muscle and skeletal injuries. DMSO is also used topically to treat painful conditions such as headache, inflammation, osteoarthritis, rheumatoid arthritis, and severe facial pain called tic douloureux.
How does a hot start work?
The hot start simply adds more air to the fuel/air mix needed to start a HOT or STALLED engine.
What is primer dimer formation?
A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached (hybridized) to each other because of strings of complementary bases in the primers.
What are the 3 steps to PCR?
PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.
What is DMSO used for medically?
DMSO is used for bladderinflammation (interstitial cystitis), limb pain that usually occurs after an injury (complex regional pain syndrome), and leakage of intravenous (IV) drug from the vein into surrounding skin and tissue (extravasation).