What is pet15b vector?
The pET-15b vector (Cat. No. 69661-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.
What is pet28?
pET28a is the most popular expression plasmid on the market (described in >40,000 published articles). It contains the T7 promoter and an adjacent lac operator sequence that is included to suppress uninduced expression5.
What is pET system?
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals, expression is induced by providing a source of T7 RNA …
What are pUC vectors?
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.
What is pGEX vector?
pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.
What does T7 polymerase do?
T7 RNA polymerase is a very active enzyme: it synthesizes RNA at a rate several times that of E. coli RNA polymerase and it terminates transcription less frequently; in fact, its transcription can circumnavigate a plasmid, resulting in RNA several times the plasmid length in size.
What T7 means?
Abstract. The late bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3′ G residue just downstream of the U run.
What does pET stand for?
polyethylene terephthalate
PET, which stands for polyethylene terephthalate, is a form of polyester (just like the clothing fabric).
What is pUC full form?
The Pre-University Course or Pre-Degree Course (PUC or PDC) is an 2-year Course under the 10+2 education system after Class 10th (SSLC, SSC) and the PUC commonly refers to Class 11th and Class 12th and called as 1st PUC (1st Year) and 2nd PUC (2nd Year) respectively in the pre-university colleges also known as Junior …
What is the primary difference between pBR322 and pUC series of vectors?
The key difference between pBR322 and pUC19 is that pBR322 is a plasmid vector that is 4361 base pairs in length, while pUC19 is a plasmid vector that is 2686 base pairs in length.
What is a pGEX plasmid?
Plasmid: pGEX-1 Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.
Why is T7 promoter important?
The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications2.
What is the T7 tag for?
The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
What is the sequence of the pet-15b vector?
The pET-15b vector carries an N-terminal His•Tag ® sequence followed by a thrombin site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map ( TB045 ). The pET Vectors are supplied as purified plasmid DNA (10 µg).
What are the pet vectors supplied with?
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information. This product is sold for internal research use only.
How can I view a plasmid collection?
As part of that effort, we supply carefully annotated files for common plasmids. Click on the links to view the plasmid collections. These combined DNA sequence and map files can be opened with SnapGene or the free SnapGene Viewer. Your time is valuable!