What kind of vector is pUC19?
cloning vector
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
What type of vector is pBR322?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
Is pUC19 a plasmid vector?
pUC19 is a commonly used plasmid cloning vector in E. The molecule is a double-stranded circular DNA (2686 base pairs in length). The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.
Is pUC19 artificial vector?
It is an artificial cloning vector created by Joachim Messing and his co-workers. It is a circular double-stranded DNA similar to the pBR322. But, pUC19 is widely used in molecular techniques due to its high copy number.
What does pUC19 mean?
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.
What is antibiotic resistance gene pUC19?
ampicillin resistance gene
In addition to β-galactosidase, pUC19 also encodes for an ampicillin resistance gene (ampR), via a β-lactamase enzyme that functions by degrading ampicillin and reducing its toxicity to the host. The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number.
What is the purpose of pUC19 in the transformation?
pUC19 control DNA (10 pg/µl) is provided to check transformation efficiency. Use experimental DNA that is free of phenol, ethanol, salts, protein, and detergents to obtain maximum transformation efficiency.
Does pUC19 have lacI?
The plasmid contains an ampicillin resistance gene (Ampr), an origin of replication that functions in E. coli, and the lacI gene, which produces a repressor that blocks the transcription of lacZ’ gene in the absence of the inducer IPTG. The complete sequence of pUC19 is 2686 base pairs.
Is Ti plasmid a cloning vector?
The Tumour inducing or Ti plasmid is present in the bacterium Agrobacterium tumifaciens. It is widely used now as a cloning vector to deliver desirable genes to the host plant to get transgenic plants.
What antibiotic resistance is pUC19?
Plasmids pT7-7 and pUC19 carry an ampicillin-resistance gene (bla), pBR322 carries ampicillin- (bla) and tetracycline-resistance (tetA) genes, pCattTrE18 carries a chloramphenicol-resistance gene (cat), pACYC184 carries tetracycline- (tetA) and chloramphenicol-resistance (cat) genes, pACYC177 carries ampicillin- (bla) …
Can pUC19 be used for expression?
pUC19 is a bacterial vector made for cloning/expression in bacteria.
How many pET vectors are there?
The improved designs are applicable to most of the 103 vectors in the pET series and can be easily implemented.
What is pUC19 vector?
Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation. • For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, see free online REviewer tool.
How many base pairs are in pUC19?
The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
Where can I find a plasmid clone for use in pUC19?
pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). NEB offers a selection of common cloning plasmids and DNAs for use as substrates. DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
What is the origin of replication in pUC19?
The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number. The high copy number is a result of the lack of the rop gene and a single point mutation in the ori of pMB1. The lacZ gene codes for β-galactosidase.