How many cells are in a 8-well chamber slide?
30,000 cells
Plate endothelial cells on the coated chamber slides at the seeding density of 30,000 cells per well for the 8-well chamber slide, or 60,000 cells per well for the 4-well chamber slide. 6.
What are chamber slides?
Removable silicone chambers on a glass slide for cell culture and immunofluorescence; suitable for upright and inverted microscopy and long-term storage.
What is glass slide used in laboratory?
A glass slide is a thin, flat, rectangular piece of glass that is used as a platform for microscopic specimen observation. A typical glass slide usually measures 25 mm wide by 75 mm, or 1 inch by 3 inches long, and is designed to fit under the stage clips on a microscope stage.
How much volume can a 96-well plate hold per well?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
How much volume can a 6 well plate hold?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Media Volume (ml) | |
|---|---|---|
| 245 mm (square) | 500 | 100-150 |
| Dishes | ||
| 6 well | 9.5 | 1.9-2.0 |
| 12 well | 3.8 | 0.76-1.14 |
How many slides are in a gross?
Slides are sold per gross. This typically means there are 144 slides (12 dozen) per box.
Why do we use glass slide in microscope?
The cover glass serves two purposes: (1) it protects the microscope’s objective lens from contacting the specimen, and (2) it creates an even thickness (in wet mounts) for viewing.
How do I stop my coverslips from bubbling?
I always centrifuge the mounting medium for 12000rpm/20min at 4 degree first. Then gently use the pipette to drop the mounting medium on one side of the slide. Then gently and slowly, particularly for brain with ventricle spaces, slide down the coverslip.
How do you seal coverslips with nail polish?
We typically dot the corners of the coverslip with nail polish, aspirate off any excess media from the edges and then finish sealing the perimeter with nail polish. We scan batches of slides overnight so they images can be processed by the time we are back in the morning.
How much does a 12 well plate hold?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Maximum Volume (ml) | |
|---|---|---|
| 245 mm (square) | 500 | |
| Dishes | ||
| 6 well | 9.5 | 16.8 |
| 12 well | 3.8 | 6.9 |
How many cells can a 6 well plate hold?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 6-well | 140675 | 1.2 x 106 |
| 12-well | 150628 | 0.5 x 106 |
| 24-well | 142475 | 0.24 x 106 |
What is the volume of a 12 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA). Approx. volume | |
|---|---|---|
| Dishes | ||
| 12-well | 150628 | 0.4 to 1 |
| 24-well | 142475 | 0.2 to 0.3 |
| 48-well | 150687 | 0.1 to 0.2 |
How much can a 12 well plate hold?
Why is 144 called a gross?
“Gross” as a noun meaning “one dozen dozen of something” is actually a shortening of the Old French “grosse douzaine,” meaning “large dozen.” This “gross” meaning 144 of something is also sometimes called a “small gross” to differentiate it from a “large gross,” which is twelve gross (1728), which is a lot of just …
What is a gross 144?
A gross refers to a group of 144 items (a dozen dozen or a square dozen, 122). A great gross refers to a group of 1728 items (a dozen gross or a cubic dozen, 123). A small gross or a great hundred refers to a group of 120 items (ten dozen, 10×12).
What is wet mount slide?
Medical Definition of wet mount : a glass slide holding a specimen suspended in a drop of liquid (as water) for microscopic examination also : a specimen mounted in this way.