What are BL21 cells?
BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins.
Why is IPTG used instead of allolactose?
Why is IPTG Better than Lactose for Induction? The bacterial cells can’t process IPTG since it is not the right substrate for the lactose metabolic pathways. Therefore, IPTG remains available in the growth medium for inducing protein expression, instead of being used up as an energy source.
Why were BL21-Gold cells used?
‡‡ The BL21(DE3) and BL21-Gold(DE3) competent cells are all-purpose strains for high-level protein expression and easy induction. When used with the CE6 bacteriophage, the BL21 and BL21-Gold cells provide the tightest control of protein expression, important for extremely toxic proteins.
What are BL21 competent cells?
BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins. For Research Use Only.
Does BL21 have kanamycin resistance?
BL21 shouldn’t have kanamycin resistance. Just to be sure, try to grow your BL21 (without plasmid) on kanamycin plates. If it doesn’t grow, it means that your selection plate do not have any BL21 (without plasmid) colonies (provided the kanamycin plate you used for selection was freshly made and the antibiotic still functional).
What is BL21 (DE3)?
BL21 (DE3) Competent E. coli 1 Ideal for routine T7 expression 2 Protease deficient B strain 3 No dry ice surcharge on competent cell shipments 4 Outgrowth medium included 5 Free of animal products More
Does BL BL21 contain Lon protease?
BL21 (DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT.